There currently is one option to travel by night bus from Rome (Italy) to Prague (Czech Republic), taking approximately 20 hours: Namely the BUS Řím - Praha operated by Regiojet.

Next offers

Regiojet operates both night trains and night buses across Europe, providing various travel options. Regiojet night trains offer a range of cabin options, from standard reclining seats in open compartments to private couchette cabins designed for one to four travelers, complete with bedding and duvets for a restful journey. Wifi is available and breakfast and coffee are served for free. Regiojet night buses feature ergonomic seating that can recline for comfort, with generous legroom and amenities such as USB charging ports, free WiFi, personal entertainment screens, and complimentary refreshments available during the journey. There is also a toilet on the bus.

For the next 7 dates with connections, the cheapest offer starts at 50 EUR. Check our connection pages below for all availabilities!

Next available offers

Wed, 30.04.2025

from 56 EUR

Sun, 04.05.2025

from 56 EUR

Wed, 07.05.2025

from 64 EUR

Sun, 11.05.2025

from 56 EUR

Wed, 14.05.2025

from 50 EUR

Sun, 18.05.2025

from 50 EUR

Wed, 21.05.2025

from 50 EUR